FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306121

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306121
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22119336
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG118150.047.85526794
CAAGTCG88850.026.2374894
TAAGTCG32350.021.93817794
GAAGTCG133450.019.19445494
AAAGTCG49850.016.12236694
GTCGGTT45600.012.9868421
GTTTATT88000.012.4977271
GTTTAAT78250.011.9527151
GTTTTTT249950.011.8651731
GGAAAAT156000.011.5391031
CGCGGAT25350.011.49506951
CGAAGAT89550.011.4416531
GTTTTAC60050.011.4271451
CGCAAAC39350.011.1080061
GGCGAAT48700.011.0985631
GTCGGGT54900.011.04371551
AACGTCG24750.010.8242439
CGCAATT22150.010.821671
AATCGGG45200.010.8141595
TCGGGTC53950.010.8025962
Produced by FastQC (version 0.11.5)