FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306122

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306122
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22119336
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG143600.037.21378794
CAAGTCG107300.023.6971194
TAAGTCG31350.020.68899594
GAAGTCG125000.017.972894
CGCGAAC10300.014.1456321
CGAAAAT37300.013.48257351
CGCGTAT4554.8432412E-813.4285721
GTCGGTT39300.013.0356241
CGAAGAT79550.012.2300431
CGCAAAC44100.012.1496591
CTTTAAT83400.011.7218231
AATCGGG41100.011.4355225
GGCGAAT37050.011.4170051
GGAAAAT154000.011.3837661
CGGAAAT44000.011.3227271
GTTTTAC60750.011.2181071
CGCGTTT15200.011.1315781
TATACGC9105.456968E-1210.8461547
GTTTTTT242100.010.6191651
AATCGTA16900.010.5680485
Produced by FastQC (version 0.11.5)