FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22841683
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG104900.052.9590194
CAAGTCG87000.028.09195594
TAAGTCG29150.024.83018994
GAAGTCG116750.019.12205794
AAAGTCG41400.014.53140194
ATCGTAT16250.012.7261546
AATCGTA22350.012.617455
GTTCGAT24300.011.60493851
GTCGTAT10600.011.5283021
CGTATCG17750.011.3859153
GTCGGAT27100.010.9261991
GGCGAAT61300.010.6574231
TTCGATT28750.010.6260872
GTCCGAT14700.010.5510221
GTCGGTT49450.010.3599611
GTCGTTT21800.010.3486241
TCGCGTA5951.5706391E-610.26890852
CGCTTAA29700.010.20707144-45
GTCGGAA34200.010.0321651
TCGGATT25800.010.019382
Produced by FastQC (version 0.11.5)