FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306128

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306128
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22566087
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG146550.042.750694
TAAGTCG32400.025.82098894
CAAGTCG113150.025.6288194
GAAGTCG131000.019.40992594
TATACGC9750.016.3897467
GTTTTTT245700.014.7484751
CGAAAAC36950.014.3734781
AAAGTCG57900.013.1502694
CGAAAAT41350.013.0713431
CGGATAT25150.012.8946331
CGCTAAT8800.012.8181831
CGCAAAC46450.012.5468241
ATATACG12050.012.0912876
GTTTTTC149250.011.9350091
TACGCTA10650.011.9154939
CTTTAAT96750.011.6589161
GTTTAAT70850.011.5426961
CGAAGAT87300.011.5211931
CTTAGAT48850.011.4493351
GGAAAAC138800.011.0727671
Produced by FastQC (version 0.11.5)