FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306129

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306129
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21646577
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG161250.061.5299294
CAAGTCG113450.032.4380894
TAAGTCG45700.029.722194
GAAGTCG165750.027.30678694
AAAGTCG58600.022.05631494
TATACGG18600.012.8870968
GTATACG20100.012.3930347
TATATCG8157.2759576E-1211.5337437
TCGCAAA19750.010.708861
ATAAGTC47450.010.59852494
GTATAGA42850.010.20078
TTACGAG31600.010.1139241
ACAAGTC122600.010.08238194
TTTTCGA41300.010.0145277
CGAAGTC80150.09.99812892-93
AGAAGTC240550.09.69112494
GCGTATA12900.09.4728676
CGCGTAA9100.09.29670288-89
TACGATT15850.09.19242994
GCAAGTC115050.09.10995192-93
Produced by FastQC (version 0.11.5)