FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306130

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306130
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21646577
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAAAC46000.014.3043471
GTTTTTT267750.014.0605041
CGGATAT22500.013.5777771
CTCGATT36050.013.428571
CGAATCG16300.013.26380353
CGGAAAT47850.013.0637411
TATACGC9000.013.0555537
CGAAAAC37300.012.8525461
TCGAATC18750.012.7839992
CGCAAAT27950.012.77996351
AAATCCA188250.012.3335974
TACGCTA9200.012.2608699
CGAAAAT47250.012.234921
CGCGAAT22150.011.8826181
AAAAATC205150.011.7986832
GTTTAAT83400.011.7218221
GGAAAAC146650.011.697921
TCGATTG23200.011.5474132
CGAAGAT79150.011.5198991
CTTTAAC74300.011.5127851
Produced by FastQC (version 0.11.5)