FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306132

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306132
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22206465
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG136400.042.6583694
CAAGTCG105050.028.63398694
TAAGTCG36550.021.60328594
GAAGTCG127900.018.99843894
TACGCTA9850.015.7461949
TATACGC9850.015.7461947
GTTTTTT289400.015.0549421
CGAAAAT47400.013.8818571
TTAGCGA21500.013.7720957
AAATCGA29700.013.6094274
AAAGTCG56350.013.5119894
CGGATAT20500.013.2975621
AAAAATC204450.013.2643692
CGCAAAC40400.012.6806941
CTCGATT41600.012.6538471
ATATACG13800.012.601456
GTTTAAT86850.012.0679331
GTTTTCT211800.011.9164311
GTTTTAT154850.011.8979671
GCGGTAT23350.011.8758041
Produced by FastQC (version 0.11.5)