FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306133
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22965255
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG91150.041.76632394
CAAGTCG72700.022.88583494
TAAGTCG29550.020.51776794
TTTTTTC426450.017.0277882
GTTTTAC72200.016.1440451
TTTTTTG471500.016.048782
TATACGC9350.015.582897
GTTTTTT277550.015.426771
GAAGTCG111800.014.46153994
TACGCTA9300.014.1505389
TTTTTTA415300.013.8974252
AAATCGA30150.013.8739644
CGCGAAT10750.013.1162811
CGAAAAC38600.012.9067361
CGGAAAT41500.012.7975921
GCGAAAT27300.012.5677661
CGAAGAT90700.012.4884241
GTTTTAT155400.012.4607481
CGAAAAT37550.012.3914791
ATATACG13350.012.3220986
Produced by FastQC (version 0.11.5)