FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306134

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306134
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22965255
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG119850.032.27451394
CAAGTCG82700.019.20918894
TAAGTCG30750.016.35447194
TACGCTA9750.015.4256419
GAAGTCG113100.015.04332494
CGCAAAC45400.014.3898691
GTTTTTT278350.014.3524351
CGAAAAC42050.013.9714621
CTTAGAT71050.013.9577771
GTTTTAC69700.013.2840761
TATACGC9600.013.2187527
TCAACGC47950.013.0364986
ATATACG11250.012.9511126
GTTTAAT83300.012.7515021
CGGAAAT42750.012.3134511
CTTAATC90850.012.2091371
GTTTTAT148450.012.1892891
CTTTAAT115550.012.0804851
CGAAAAT41650.012.0744311
CGCAAAT35100.012.0512831
Produced by FastQC (version 0.11.5)