FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306135

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306135
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22610902
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG110100.055.6657694
CAAGTCG94050.029.13450494
TAAGTCG28450.024.2847194
GAAGTCG116700.019.65381494
AAAGTCG48050.014.7700394
GTCCGAT15800.014.575951
GTCGGAT29950.012.5542571
GTCGGAA39600.011.9873731
GGCGAAT44250.011.6836171
TCGGATT26750.011.5962622
AATCGTA18450.011.4634155
GTCGGTT49350.011.2380951
GTCGAAA31050.010.5958131
CGCGTAA8159.058567E-1010.3803681
CGTATCG17800.010.2977523
GGCGATT41850.010.2198321
GTTCGAT33450.010.1165921
CGTCGGA24350.010.03696294
GCGGTAT24250.09.8845361
TTAGCGA20050.09.84538658
Produced by FastQC (version 0.11.5)