FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306136

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306136
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22610902
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG132000.046.25227494
CAAGTCG111600.027.50089694
TAAGTCG28650.024.11518394
GAAGTCG120500.017.35684694
CGCGTAT4153.4067834E-612.4578321
GTCCGAT15000.011.2800011
CGTATCG19350.011.1731273
TTAGCGA24900.011.1365478
GTTCGAT33000.010.8242431
AAAGTCG51700.010.72727394
GGGAAAT152950.010.4785881
GCCGGTT53450.010.4639851
TAGCGAG29700.010.4444459
CGCGGAT25750.010.4038831
GGCGGAT93650.010.3886811
GCGGTAT25150.010.0914521
GTCGGGT47900.09.910231
TATACGG15250.09.86229534-35
AATCGTA19150.09.8172325
TTCGATT34050.09.8002932
Produced by FastQC (version 0.11.5)