FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306137

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306137
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21925929
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG142350.064.0533994
CAAGTCG113000.033.85663694
TAAGTCG38350.026.7170894
GAAGTCG149650.025.4079594
AAAGTCG57500.019.7808794
GCGAAAT28650.018.701571
TTTTTTC362800.015.5716652
GTTTTTT277400.015.11319351
GTCGATT22300.014.7533641
GCGGAAT40100.013.8304241
GCGGTAT26600.013.6052631
GCCGAAT51800.013.1563691
GAAAAAT205150.013.0816491
CGAAAAT36350.013.0591471
AAATCCA178900.012.7680264
AATCCAC107700.012.6555245
GTTTTTC154500.012.5637541
TTTTTTG411200.012.5615282
CGCAAAT29600.012.3851361
CTCGATT40150.012.1743471
Produced by FastQC (version 0.11.5)