FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306138
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21925929
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG174450.052.2671294
CAAGTCG136750.030.55429894
TAAGTCG39700.030.1889294
GAAGTCG145700.023.48387194
AAAGTCG65400.016.16972494
GCCGAAT48750.014.750771
GCGAAAT28750.014.22260951
CGCGTAT4755.702532E-913.8526311
CGCAAAT29550.013.3604071
GTTTTTT269150.013.2364851
AAATCGA31650.013.067934
GAAAAAT192500.011.939221
GTTTAAT78500.011.914651
CGCAAAC41150.011.8784941
AAATCCA178500.011.7697484
CGAAAAT40850.011.7356191
AAAAATC187600.011.6247332
CGGATAT21450.011.3939391
CGCGAAC18200.011.3626371
GTTTTAT142500.011.11508751
Produced by FastQC (version 0.11.5)