FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306140

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306140
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22832979
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG107800.032.35064794
CAAGTCG83100.020.58724294
TAAGTCG28400.017.8732494
GTCGGTT44300.014.2167031
GAAGTCG100950.013.3620694
CGCGAAT10050.012.1592041
CGAATCG16850.011.7151333
CGTAACG5758.211828E-811.4434773
CGGAAAT46750.011.3604281
TCGGATT28300.011.2932852
AATCGGG35650.011.0743335
CTCGTTT41100.010.9781021
CGAAGAT93400.010.9700211
GGAAAAT175850.010.9582031
GTGAATT98050.010.8332471
CGCGTTT14550.010.6597931
CGCAATT24000.010.5749991
GTTTATT106650.010.4444441
TTAGCGA17300.010.32378
CGAGAAT34750.010.1438841
Produced by FastQC (version 0.11.5)