FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21453578
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG128200.059.24492694
CAAGTCG97500.031.09230694
TAAGTCG35000.028.06571494
GAAGTCG141500.021.98869194
AAAGTCG49550.017.73763894
GTTTTTT285500.015.1782831
TTTTTTC357000.014.0341732
TTTTTTG382250.012.8612172
GTTTTCT210950.011.2737611
GTTTTAC59850.011.1512111
GTTTTAT152700.011.1113281
GCGAAAT27100.011.099631
GCGGTAT23550.010.97664451
CGAAAAT35400.010.8870051
AAATCCA181000.010.7762434
AATCCAA98600.010.7251525
GTTTAAT85500.010.7192971
GAAAAAT193200.010.7039341
CGGAAAT40850.010.7001231
GGAAAAT159900.010.6991861
Produced by FastQC (version 0.11.5)