FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306142

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306142
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21453578
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG154300.050.01555394
CAAGTCG110300.027.7824194
TAAGTCG37500.025.81866594
GAAGTCG139800.021.18025694
AAAGTCG58750.016.3294
GTTTTTT286600.013.11932851
CGAAAAT42500.011.6117641
GTCGGAT38800.011.26546394
CGAAAAC32600.011.24539851
CGCGTAT4601.0732081E-511.239131
CGCAAAC43700.011.0778031
GTTTAAT79350.010.9577811
AAAAATC187850.010.7585842
CGCGAAC15800.010.7088591
CGGATAT22450.010.6770591
CGCGAAT14300.010.5174821
GGAAAAT160200.010.4737821
CGGAAAT44000.010.4681811
AAATCCA182100.010.4530484
AAATCGA32000.010.4281244
Produced by FastQC (version 0.11.5)