FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22786497
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG112950.051.764594
CAAGTCG92150.027.89907894
TAAGTCG31550.020.70681694
GAAGTCG128500.018.76342694
AAAGTCG48350.016.4281394
TTTTTTC363350.015.8197342
TATACGC10200.015.6666677
GTTTTTT253750.015.5400991
CTTAGAT74200.014.75875951
CGCGAAT9950.014.17085551
CGAAAAT33950.014.1207681
TTTTTTG406950.013.5935622
ATCAACG50950.013.4681065
TACGCTA11300.013.3097349
CAACGCA53700.013.1284927
CGAATCG17600.013.0852273
ATTAAGC65750.012.8669216
CTCGATT56400.012.751
GGAAAAT154500.012.6854361
GTTTAAT84700.012.6517121
Produced by FastQC (version 0.11.5)