FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306144

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306144
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22786497
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG151600.038.2572694
CAAGTCG105350.024.49264594
TAAGTCG36450.022.17832694
GAAGTCG134600.017.87815994
TATACGC11550.014.649357
TACGCTA12000.014.4916699
CGAATCG16250.014.4615383
CGAAAAT38450.014.3016921
CGCGTTT14750.013.7016961
GTTTTTT268150.013.6714531
GTTTTAC65150.013.2739841
ATATACG14150.012.9540656
CGAAAAC44450.012.7941511
CTTAGAT73450.012.6698441
AAATCCA179900.012.3574224
CGCGAAT9200.012.260871
AAAAATC178400.012.2505622
GGAAAAT156850.012.1957281
AATCGTA18900.012.1851855
TCAACGC51350.012.1733216
Produced by FastQC (version 0.11.5)