FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306145

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306145
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21300682
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG177350.066.809794
CAAGTCG133400.032.90704794
TAAGTCG50650.030.25074294
GAAGTCG168300.029.2388694
AAAGTCG63000.024.6190594
TATACGG15700.011.9745238
ACAAGTC135750.011.49465994
GTATACG17250.010.6260887
GCAAGTC127400.010.624804594
CGAAGTC80150.010.6138594
ATAAGTC60050.010.48792794
AGAAGTC254400.010.05031594
CGTATCG16750.09.96119436-37
TCGCGTA6950.09.80575786-87
CGATAAT31650.09.8009484
TTACGAG27500.09.2290921
TAGCGTA8702.8967406E-89.1839084
TTAAGTC73750.08.79457794
CGCGTAA6253.274181E-118.64800188-89
CTTCGAT37800.08.5793661
Produced by FastQC (version 0.11.5)