FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306152

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306152
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22879461
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG146900.048.98366594
CAAGTCG115300.028.49349694
TAAGTCG35150.025.80654594
GAAGTCG135650.020.99668594
GCGAAAT28650.016.8970361
AAAGTCG60850.016.14297594
AAAAATC246300.014.4644752
GCCGATT27700.014.4223831
GTTTTTT340600.014.130361
GCGGAAT46100.013.2537981
AAATCGA35900.013.2228424
AATCGAG36900.012.8644995
GAAAAAT247400.012.7093781
TCGAATC19100.012.5497382
CGCGAAC13200.012.4621221
AAATCCA216800.012.4003714
CGGATAT19250.012.2077921
CGCAAAC44300.011.7765251
AATCCAC120900.011.7402815
CTCGCAT50450.011.7383561
Produced by FastQC (version 0.11.5)