FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22497030
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG116450.054.9308794
CAAGTCG93050.027.12412694
TAAGTCG35700.025.93557494
GAAGTCG126900.020.85185294
AAAGTCG47250.018.8994794
CGAAAAT34400.012.7063961
CGGAAAT45300.012.6578361
CGCAATT21150.012.2222221
GTCGGTT43850.012.1117451
GGAAAAT201650.011.4673941
CGCGAAT13750.011.281
GTTCGAT25250.011.1683161
GTTTTTT305550.010.8751441
GTTTATT114300.010.8145231
CGCAAAT27300.010.6739931
CGAAGAT75350.010.6662251
GTTTTAT157700.010.4908051
CGCGCAT17250.010.081161
CTCGGAT61950.010.0145281
CTTTAAT111600.09.9390681
Produced by FastQC (version 0.11.5)