FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306155

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306155
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23106606
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG99250.051.0015194
CAAGTCG80400.026.3644394
TAAGTCG29500.022.46440794
GAAGTCG113250.018.0114894
GTCCGAT14900.015.4563761
GTTCGAT34800.014.1810331
AAAGTCG43500.013.28965694
TTCGATT35650.013.1837322
CGTAACG4608.033712E-712.2608693
CGTTCGA17300.011.9537577
CTCGTAT18000.011.751
CTCGGTT41750.011.7077851
CGCGTAA11450.011.493451
TATACGG17000.011.19705934-35
GTCGTTT20200.011.1683161
CTCGTTT41950.010.6436221
GTCGGTT46000.010.4217391
CTCGGAT65650.010.30921651
ACGTTCG23350.010.064246
TCGGATT28800.09.9548612
Produced by FastQC (version 0.11.5)