FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306156

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306156
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23106606
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG127550.039.3171394
CAAGTCG97550.022.25935694
TAAGTCG30700.019.59609294
GAAGTCG119150.017.55350594
GTCCGAT15900.015.3710691
GTTCGAT35950.013.8581361
CGCGTAA8500.013.8235291
TTCGATT37350.013.3386892
TATACGG16250.012.29230834-35
CGTATCG16600.011.6084343
GTTTATT114500.010.8777291
CTCGTAT18400.010.4728251
CGATTGG16750.010.382094
AAAGTCG51750.010.35362394
TCGATTC40650.010.2902833
GGGAAAT168200.010.25505351
TCGGATT27050.010.2513872
AATCGTA18750.010.0266675
GTCGGTT45000.010.0266661
CTCGTTT40350.010.0173491
Produced by FastQC (version 0.11.5)