FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306157

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306157
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21880936
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG246700.057.325994
CAAGTCG176450.031.53754694
TAAGTCG53350.028.98406894
GAAGTCG211500.027.494
AAAGTCG71450.021.31280794
TATATCG8050.014.5962727
TTACGAG30200.012.9172181
GCAAGTC145150.012.77402692-93
TATACGG16950.012.4778768
CGAAGTC91300.012.40635394
ACAAGTC139750.012.14096694
GTATACG17400.011.8850587
ATAAGTC51150.011.2561192-93
AGAAGTC261550.010.08105694
ACGGAAC43850.010.0752563
CGTATCG22750.09.91648436-37
TTAAGTC69000.09.46811794
TAGCGTA10457.4578566E-119.4449764
TACGATT16700.09.28742594
GCGTATA11656.91216E-118.8755376
Produced by FastQC (version 0.11.5)