FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22177576
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG99050.049.6335294
CAAGTCG80450.026.4064694
TAAGTCG27100.023.23985194
GAAGTCG115700.017.58945594
TATACGC8350.016.3233537
GTCGGTT43300.014.5450331
TACGCTA8450.014.4615369
CGGAAAT45800.013.34061051
ATATACG10850.012.9953916
CGAATAT12750.012.5333331
CTTTAAT91400.012.2385111
GTTTAAT87250.011.7971351
GTCGGGT53000.011.7943391
CGAAAAT30850.011.7309551
AACGTCG30350.011.6144969
GTTTATT95500.011.4670141
CGAAGAT87350.011.3531761
GTTTTTT257450.011.1361431
CGAGTAA31250.011.1295994
CGCAATT21900.010.9452051
Produced by FastQC (version 0.11.5)