FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21867306
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAT29250.015.2649561
GTTTTTT283200.014.4551541
CTCGATT31750.013.9149611
CGCAAAC41900.013.460621
AAAAATC197700.012.885182
CGAAAAT42800.012.4088781
GTTTAAT84150.012.3434341
GGAAAAT166250.012.1846631
TTTAATC103200.011.9777132
GAAAAAT211050.011.8919691
AAATCCA187100.011.8567614
GCGGAAT37250.011.7342281
GTTTTAT150650.011.6681041
CGAAAAC30800.011.5974031
GGCGAAT31650.011.4344391
GCGATTT33300.011.4324331
AAATCGA32850.011.1598184
AAAATCC168000.011.0785713
TCGAATC19750.010.94683552
GGAAAAC143000.010.9118881
Produced by FastQC (version 0.11.5)