FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306164

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306164
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22186796
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG65500.013.05954194
TTAGCGA18300.012.58478
CGGGAAT51850.011.3307621
GTCGGTT42150.011.1506521
GTCCGAT13700.010.9781021
GTTCGAT24000.010.9666661
GGCGGTT55100.010.8330311
CGCGTAA5354.9391765E-610.5420561
TAGCGAG23500.010.1999999
CGCGTGT25650.010.0779721
CGGAAAT42200.09.9123221
GGGAAAT152050.09.8605711
CGGGATT39450.09.7693291
GGCGAAT31300.09.7603841
CGGTTGT47350.09.23125653
GTCGGTA15900.09.1635221
CGCTTAT21050.09.1543941
CGCGGTT39050.09.1472471
CGGGGAT72350.08.9647541
GTTTATT122850.08.9523811
Produced by FastQC (version 0.11.5)