FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22603356
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG165200.053.91343794
CAAGTCG129450.030.17149494
TAAGTCG40200.025.95522594
GAAGTCG143450.024.90066194
AAAGTCG64150.018.60950794
GCGAAAT27050.015.6377071
GTTTTTT307050.014.0517831
CGAAAAC33300.013.9729731
AAATCCA211400.013.0950794
CGGAAAT43450.013.0886071
AAATCGA36450.013.0233194
CGAAAAT41800.012.5933011
AATCGAG36600.012.58475
AAAAATC228750.012.4511472
GTCGATT19650.012.4376591
GCCGAAT47600.012.1449581
CTCGATT38550.011.9481181
AATCCAC120900.011.6236555
CGCAAAT30000.011.5933331
GTTTTTC174650.011.3026051
Produced by FastQC (version 0.11.5)