FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22717301
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG97050.052.20607894
CAAGTCG81950.027.29957694
TAAGTCG27500.022.9018294
GAAGTCG114250.018.30634594
AAAGTCG41750.014.1844394
AATCGTA23950.012.1670155
GTTCGAT23100.012.0043291
GTCCGAT16650.011.8558571
GTCGGTT48100.010.8461541
GTCGGTA17600.010.6818181
GTCGGGT38400.010.64843751
ATCGTAT19500.010.6051286
GTCGAAT13000.010.1230761
GGGAAAT170700.09.9947271
GGGGAAT130400.09.9478521
GGCGAAT41950.09.8593561
GTCGAAA30150.09.6650091
CTCGGTT37150.09.6150741
GTCGGAT24050.09.5758841
CGCGTAT5204.5431894E-49.0384621
Produced by FastQC (version 0.11.5)