FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306173

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306173
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22615877
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG94950.047.96524494
CAAGTCG75950.026.67149494
TAAGTCG32950.023.67830394
GAAGTCG112950.017.26870394
CGGAAAT42200.013.364931
CGCAATT20200.012.79702951
AAAGTCG44250.012.63954894
GTCGTAT11900.012.6386571
TACGCTA7501.6370905E-1111.9066679
GTTTTTT318900.011.8642221
GGAAAAT203950.011.6376561
AAATCGA35750.011.5692324
GTTTATT115400.011.4038131
TATACGC7854.0017767E-1111.3757977
GTCGGTT42350.011.3199541
CGCGAAT9700.011.1443311
GTTTTAT165300.010.918331
CGCGTTT14800.010.79729751
CGCAAAT33250.010.6015041
CGAAAAC34750.010.5496411
Produced by FastQC (version 0.11.5)