FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23331813
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG124300.058.38133694
CAAGTCG99600.030.38955794
TAAGTCG37450.025.47663594
GAAGTCG148150.022.04859794
AAAGTCG49200.016.90853794
GTTTTTT293850.014.6030281
TTTTTTC383200.013.5775052
TTTTTTG398100.013.458932
GTTTTAC67850.012.8843031
GTTTAAT90900.012.09900951
CGAAAAC33100.012.0694871
CGAAAAT38600.011.8108821
CGGATAT24050.011.5301461
GGAAAAT194150.011.4988411
GCCGAAT53750.011.4548841
AAATCCA199400.011.3375134
CGCAATT22500.011.281
GGAAAAC151850.011.2044781
AAAAATC217950.011.0841932
GTTTTAT159100.011.0483981
Produced by FastQC (version 0.11.5)