FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23331813
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG143450.050.8170194
CAAGTCG113450.029.66240794
TAAGTCG37550.025.0332994
GAAGTCG138950.021.30982494
AAAGTCG54850.017.0519694
CGAAAAT46300.014.6177111
GTTTTTT291600.013.9420441
AAATCGA33750.011.9762974
CGAAAAC34250.011.8014591
GTTTAAT87450.011.4476851
CGCGAAT7008.1308826E-1011.4142871
GGAAAAC154200.011.338521
GGAAAAT190500.011.1270331
CGCAAAC47550.010.8727661
CGCGGAT25200.010.8174611
GTCGAAT15350.010.7166131
CGGAAAT42000.010.5190471
AATCCAC123550.010.49939255
AAATCCA206500.010.4924944
CGAATCG15350.010.4104233
Produced by FastQC (version 0.11.5)