FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306183

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306183
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22419596
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG107950.052.72533494
CAAGTCG88150.027.13896894
TAAGTCG31550.024.72994
GAAGTCG117000.020.64786194
AAAGTCG41400.014.98550794
CGTTCGA16000.012.6312487
ATCGTAT15900.011.2327046
GTTCGAT29850.011.0217751
ACGTTCG21950.010.9202726
GTCGGTT46850.010.2326571
GTCGAAT14250.09.8947361
TTAGCGA19050.09.8687668
GTCGTTT20100.09.8208951
TATACGG14400.09.79166534-35
CTCGGTT38900.09.7866321
CGCGTGT23250.09.5010751
CGCATTT29750.09.47899151
TAACGCA21850.09.46453152-53
GTTTATT102600.09.4366461
CGCGTAA7603.7501013E-79.2763161
Produced by FastQC (version 0.11.5)