FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306184

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306184
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22419596
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG133350.043.03486694
CAAGTCG100150.025.10733894
TAAGTCG28750.022.5694
GAAGTCG125000.018.42399894
CGCGTAA9000.015.14444351
AAAGTCG52700.013.1992494
GTCGTTT25250.011.7267321
ATCGTAT16550.011.3595176
CGCGTAT5404.503272E-711.3148141
AATCGTA20150.011.1960295
CTCGTAT15300.010.4444441
GTTTATT109650.010.4158681
GTCGGAT33250.010.31879794
CGCATTT27600.010.0471011
GTTCGAT31850.010.0345361
CGTATCG16850.09.7626113
CGTAACG4409.998187E-49.6136363
GGCGGTT55450.09.4932371
CGCATAT10901.8189894E-119.48623851
TAATCGT18850.09.4748014
Produced by FastQC (version 0.11.5)