FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306191

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306191
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22340722
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCTA7300.018.0273979
CTCGATT43350.017.4555951
TTTTTTC538000.017.0527862
GTTTTTT373650.016.7798751
TATACGC9200.015.3260877
CGAGAAC63200.014.9477841
TTTTTTG580000.014.6105172
TTTTTTA607650.014.4639172
CGCAAAT33250.014.4180451
GTTTTAC93700.013.9946631
CGGATAT24700.013.7004051
AAATCCA225150.013.6313574
TCGATCC21850.012.9061782
CGAAAAT42750.012.7532171
ATATACG13900.012.5107926
CGAAAAC39100.012.5012791
CTTTAAC96350.012.4878041
AAGTCGG46050.012.3496294
GTTTAAT121800.012.2709361
CTCAGAT173650.012.260871
Produced by FastQC (version 0.11.5)