FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306192

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306192
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22340722
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTTTT385050.016.2342551
TACGCTA9050.016.0994479
CGCAAAT31950.015.1517991
CTCGATT50050.015.0249751
TATACGC11650.014.9270397
CGAGAAC56650.014.3530451
CGAAAAC40300.013.7617871
CTCAGAT176450.013.5046761
CGAAAAT51150.012.6803521
CGCAAAC39500.012.61265851
AAATCCA222300.012.4952774
CGGATAT22850.012.3413561
TTTTATC151450.012.1030042
GTTTTAT210350.011.9315421
ATATACG13850.011.8772566
CTTAGAT85050.011.8259861
CGAAGAT90750.011.8082651
CGAATCG14350.011.7909413
CGGACAT50250.011.598011
CGGAAAT45950.011.455931
Produced by FastQC (version 0.11.5)