FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306193

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306193
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22224168
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG154500.057.3430494
CAAGTCG111400.031.64272994
TAAGTCG42450.026.35194
GAAGTCG160650.026.12573694
AAAGTCG52200.018.6379394
TATACGC13200.017.0909087
GTTTTTT273250.016.8391571
ATATACG15750.016.7111116
CGCGAAT15300.016.5882341
TACGCTA13550.016.3025829
TTTTTTC356000.016.1859552
CGCGAAC10500.015.2190471
CGCAAAT35600.014.9185391
GTTTAAT80500.014.5378881
CGAAAAT30600.014.2843131
CGAAAAC41250.014.1284851
TTTTTTG367750.013.9562192
GCGAAAT30500.013.7147531
CGCAATT20050.013.3615961
GTTTTAC63600.013.2279861
Produced by FastQC (version 0.11.5)