FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306197

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306197
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22992686
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG98000.052.3714394
CAAGTCG75400.027.1777294
TAAGTCG32900.023.5714394
GAAGTCG114750.019.41438194
CGCGTAA9900.012.8181831
AAAGTCG45350.012.64388294
CGTTCGA17050.012.1290337
GTCCGAT16750.011.7850751
AATCGTA24900.011.5140575
CGCGAAT9950.011.3366831
CGCTTAA40950.011.13308944-45
GTTCGAT29250.010.92649651
ACGTTCG21450.010.7365986
GGCGATT39800.010.628141
TTAGCGA20800.010.3942318
GGCGAAT56100.010.2210341
GTCGGAG34200.010.0321651
CTCGTAT19700.010.0203051
CGCGTAC16450.010.0000011
GGGAAAT175600.09.9031891
Produced by FastQC (version 0.11.5)