FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22992686
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG122450.042.06778394
CAAGTCG95000.024.73684394
TAAGTCG32450.021.1463894
GAAGTCG110800.018.79151794
CGCTTAA38550.012.92347644-45
CGTATCG19700.012.4060923
AATCGTA24300.011.4115245
AAAGTCG49200.010.79471594
CGCGTAT5253.941479E-610.7428581
GTCCGAT15850.010.6750781
CGCGGAT22750.010.5362631
GTTCGAT30550.010.4615391
CGGGAAT53300.010.1407141
CCCGTAT33000.010.1121231
GTTTATT127900.09.9218141
CCGTATC32550.09.8187412
TTAGCGA18750.09.7760018
CGCTAAT10103.8198777E-119.7722781
CACGCTT52450.09.72259342-43
CGGGGTT90950.09.5085221
Produced by FastQC (version 0.11.5)