FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306201

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306201
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22333502
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG127500.055.4784394
CAAGTCG95800.030.02505394
TAAGTCG35250.028.66666494
GAAGTCG138350.022.21756494
AAAGTCG51000.017.3254994
GTTTTTT291600.015.7311381
TTTTTTC384450.014.7803352
TTTTTTG400750.013.1822842
CGAAAAT35900.013.0919221
CGCAAAT30500.012.94426251
GCGAAAT32800.012.4664631
CGAATCG16800.012.0297613
GTTTAAT96800.011.9442151
TTAGCGA21700.011.9124437
TTTCGCG12700.011.842528
GTTTTCT214850.011.7910181
GGAAAAT166850.011.7464791
TTTTTTA368400.011.7117262
CGAAGAT78500.011.2560511
AAATCCA186700.011.1521164
Produced by FastQC (version 0.11.5)