FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306204

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306204
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22526311
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG155850.054.01154794
CAAGTCG131650.030.06000994
TAAGTCG37500.028.19999994
GAAGTCG139600.020.87392694
AAAGTCG56050.015.34522794
CGCGTAT5402.4538167E-913.0555551
TTAGCGA20450.011.0317848
CTCGTAT14700.010.5510211
GGCGAAT40900.010.2273831
GTTCGAT28500.010.2245621
GGCGATT37050.010.1484481
TAGCGAG24100.010.1410799
GTCGTTT22350.010.093961
TACCGTA19450.09.665819
GCCGGTT48350.09.526371
CTCGGAT58900.09.4957551
CGGAAAT42350.09.4332941
ACAAGTC134600.09.42793594
GCGGTAT22450.09.4209361
GGGAAAT153700.09.1431371
Produced by FastQC (version 0.11.5)