FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306205

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306205
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22421897
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG202050.059.31794
CAAGTCG142900.032.42967294
GAAGTCG193800.027.18627794
TAAGTCG56100.026.8092794
AAAGTCG65850.020.41306194
TATACGG18650.012.0965158
GTATACG18550.011.9083577
TTACGAG34350.011.082971
CGAAGTC89900.010.71746494
ACAAGTC143450.010.58278294
TACGATT20300.010.4187294
GCAAGTC136650.010.18075594
ATAAGTC62000.010.15806694
AGAAGTC259800.09.84141794
TATGAGT63450.09.7777795
GGTGTAT70600.09.0538251
CGTAGCG34300.09.04373316-17
GAATCGG36400.08.97390122-23
TACGAGT43250.08.5849722
GTATAGA47400.08.5274278
Produced by FastQC (version 0.11.5)