FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306206

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306206
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22421897
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAT24450.014.6094071
GTTTTTT303750.014.3746511
CGCAAAC41800.013.8301441
CGAAAAC35650.013.5792441
TATACGC8200.013.1829267
CGAAAAT47150.012.8589611
TTAGCGA18650.012.6005367
AAAAATC225350.012.4721552
CTCGATT37350.012.4578321
CGGAAAT48400.012.3326451
CTCAGAT168200.012.2948881
AAATCGA35500.012.0478874
TACGCTA9450.011.9365089
AAATCCA206100.011.8811264
CGGAAAC62900.011.6565981
GAAAAAT229800.011.6375111
GTTTTTC169600.011.5283031
GTTTTAT157150.011.3948461
GTTTTAC73500.011.3183681
CGCGAAC10800.011.3148151
Produced by FastQC (version 0.11.5)