FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306209

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306209
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22327620
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG98750.054.1630494
CAAGTCG79650.026.37664894
TAAGTCG32150.022.36703194
GAAGTCG115900.018.81622194
AAAGTCG46350.014.5005494
CGGAAAT41750.012.2706591
CGCAAAT33950.012.0441831
GTCGGTT45750.011.9169391
CGAAAAT32050.011.731671
CGAAAAC30150.011.6915431
CGAATCG14900.011.6711413
GTTTAAT97900.011.6659861
AATCGGG38200.011.5654465
GGAAAAT196800.011.4634141
GTCGAAT15200.011.4407891
CGAAGAT98900.011.3104141
GTTTTTT324150.010.8745951
CGCGGAT23050.010.8069421
GTTTTCT236450.010.6741381
GTTTTAT158200.010.6656121
Produced by FastQC (version 0.11.5)