FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22327620
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG122450.043.52633794
CAAGTCG89550.023.14573194
TAAGTCG36550.020.9603394
GAAGTCG117850.018.18583194
AAAGTCG50700.014.5542494
CGAAAAT44450.011.9482571
CGGAAAT42500.011.9435281
GTCGGTT43000.011.5860461
CGAAGAT91350.011.3705541
CGAATCG15250.011.0950823
CGCGTTT16100.010.8012431
CGCAAAC41000.010.775611
AAATCGA36200.010.6464084
GGAAAAT189850.010.5462211
GTTTTTT306900.010.4444441
CGCGAAC14950.010.3745821
CGCAAAT31650.010.2464461
CTCAGAT174200.010.1446621
CTTTAAT117900.010.0856661
AATCGGG33300.010.0210215
Produced by FastQC (version 0.11.5)