FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23127590
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG99250.052.280194
CAAGTCG78250.025.5872294
TAAGTCG32650.021.01684494
GAAGTCG115400.019.71230594
AAAGTCG51800.013.06563894
CGCGGAT22550.012.2971171
GTTCGAT30200.011.8278141
TCGGATT26500.011.705662
AATCGTA30300.011.4785475
TTAGCGA18950.011.4089728
CGCTTAA34050.010.97356844-45
GTCCGAT15200.010.8223681
GGGAAAT173050.010.7009531
TTCGATT32800.010.6036592
TCGGGTC52450.010.3050532
GTCGAAT15550.010.2765261
ATCGTAT22200.010.1621626
CTCGGTT37950.010.031621
CGGGGTT91100.010.00878051
CGCAAAT27650.09.8589511
Produced by FastQC (version 0.11.5)