FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306213

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306213
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22227963
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG58400.026.79965694
GTTTTTT352950.016.8717941
CGCAAAC43100.014.2853811
TAAGTCG23050.013.66160594
CGCGAAT11050.013.6108591
GTTTAAT113150.013.5413151
CAAGTCG46250.013.51567694
TTTTTTC451800.013.4924752
CGAAAAC33350.013.1064471
TTTTTTA494600.012.9425792
CGCATAT9550.012.7958111
CTCGATT48450.012.7079461
TTTTTTG502350.012.6306352
GTTTTTC180100.012.6046641
CGCAAAT31850.012.5431711
GGAAAAT196850.012.4632971
CGTATCG16900.012.37573950-51
CTCAGAT176800.012.3614241
GTTTTCT239550.012.3018161
GTTTTAT192700.012.2682911
Produced by FastQC (version 0.11.5)