FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005307400

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005307400
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23128408
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT5806.0645107E-912.2855541
CGTATCG21200.011.2078593
CGACCTA16550.09.7626474
CCGGGAT67050.09.6354421
CGCGAAT11705.456968E-129.3384211
CCCGTAT33200.09.1574251
CCGGAAT51200.08.9998071
CCGGCAT68650.08.9956871
GTATCGA33450.08.9501664
CTCGAAT49450.08.9340451
CGCGATT11252.6193447E-108.8674391
CTCGGTT45950.08.7874911
CGCCAAT41750.08.7612431
CGAAGAT91900.08.6324181
GATCGGA92100.08.50789794-95
CGGCAAT46350.08.5066751
CGCGTTT20850.08.4299921
CGGACAT61850.08.3717891
CGGGAAT60300.08.2718651
CGCGTGT28150.08.2689281
Produced by FastQC (version 0.11.5)