FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005307405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005307405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20883608
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTA17700.016.6371634
CGTATCG22400.013.7824753
CGACCGT15250.012.7824751
GTATCGA26200.011.96474554
TACGCAC22250.011.5263939
CCCGTAT32300.010.3037761
CTCGACT30850.09.8633791
CGCGAAA11700.09.7430342-43
CGGGAAT51150.09.5739791
CTTGGAT161550.09.270531
CGAAGAT74600.09.1775041
CGCCAAT41450.09.1762731
CTCGGAT69450.09.0365491
CCGGCAT56900.09.0242771
CTCGGGT59600.08.934551
GATCGGA78200.08.89897794-95
CCGAAAT38500.08.8914521
CCGCAAT30500.08.8853791
CCGTATC39200.08.849342
CGCGAAC10801.1204975E-98.804551
Produced by FastQC (version 0.11.5)