FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005307406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005307406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20883608
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTA19150.015.1249544
CGCGTAA4505.429665E-712.6639851
CGTATCG24450.012.2345923
TACGCAC22200.011.5467959
GTATCGA26050.010.754184
CCCGTAT32300.010.5859941
CGAAGAT74500.010.19918251
CGGGTAT22050.09.9071991
CTCGGAT67250.09.745151
CCGGTAT40150.09.6990671
CCGAGAT88700.09.4230341
GATCGGA78100.08.97648894-95
CGACCGT14300.08.9666331
CGACAAT30200.08.9633341
CTTGGAT156450.08.89392951
CGCCAAT41300.08.6240821
CCGGAAT52400.08.6098181
CCGGGAT59100.08.598011
CGCGACT12201.4370016E-108.5637611
CGGGAAT51200.08.533351
Produced by FastQC (version 0.11.5)